Phosphorylase
Identifiers
EC number	2.4.1.1
CAS number	9035-74-9
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Phosphorylases are enzymes that catalyze the addition of a phosphate group from an inorganic phosphate (phosphate+hydrogen) to an acceptor.

A-B + P ⇌ A + P-B

They include allosteric enzymes that catalyze the production of glucose-1-phosphate from a glucan such as glycogen, starch or maltodextrin. Phosphorylase is also a common name used for glycogen phosphorylase in honor of Earl W. Sutherland Jr. who in the late 1930s discovered the first phosphorylase.^[1]

Function[edit]

Phosphorylases should not be confused with phosphatases, which remove phosphate groups.
In more general terms, phosphorylases are enzymes that catalyze the addition of a phosphate group from an inorganic phosphate (phosphate + hydrogen) to an acceptor, not to be confused with a phosphatase (a hydrolase) or a kinase (a phosphotransferase). A phosphatase removes a phosphate group from a donor using water, whereas a kinase transfers a phosphate group from a donor (usually ATP) to an acceptor.

Types[edit]

The phosphorylases fall into the following categories:

• Glycosyltransferases (EC 2.4)
  
  
  
  • Enzymes that break down glucans by removing a glucose residue (break O-glycosidic bond)
    
    
    
    • glycogen phosphorylase
    
    
    • starch phosphorylase
    
    
    • maltodextrin phosphorylase
    
    
    
    
  
  
  • Enzymes that break down nucleosides into their constituent bases and sugars (break N-glycosidic bond)
    
    • 
      Purine nucleoside phosphorylase (PNPase)
    
    
  
  
  
  


• Nucleotidyltransferases (EC 2.7.7)
  
  • Enzymes that have phosphorolytic 3' to 5' exoribonuclease activity (break phosphodiester bond)
    
    
    
    • RNase PH
    
    
    • 
      Polynucleotide Phosphorylase (PNPase)
    
    
    
    
  
  

All known phosphorylases share catalytic and structural properties [1].

Activation[edit]

Phosphorylase a is the more active R form of glycogen phosphorylase that is derived from the phosphorylation of the less active R form, phosphorylase b with associated AMP. The inactive T form is either posphorylated by phosphoylase kinase and inhibited by glucose, or dephosphorylated by phosphoprotein phosphatase with inhibition by ATP and/or glucose 6-phosphate. Phosphorylation requires ATP but dephosphorylation releases free inorganic phosphate ions.

Pathology[edit]

Some disorders are related to phosphorylases:

• 
  Glycogen storage disease type V - muscle glycogen


• 
  Glycogen storage disease type VI - liver glycogen

See also[edit]

• Hydrolase

References[edit]

External links[edit]

• Muscle phosphorylase deficiency - McArdle's Disease Website


• 
  Phosphorylases at the US National Library of Medicine Medical Subject Headings (MeSH)